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验证数据展示

  • WB analysis using 68489-1-Ig

    WB analysis using 68489-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68489-1-Ig (OTUB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of U2OS using 68489-1-Ig

    IF Staining of U2OS using 68489-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using OTUB1 antibody (68489-1-Ig, Clone: 2F7F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

OTUB1 Monoclonal antibody

OTUB1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68489-1-Ig

产品说明书

CloneNo. 2F7F8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

OTU domain-containing ubiquitin aldehyde-binding protein 1, OTB1, HSPC263, hOTU1, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68489-1-Ig targets OTUB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OTUB1 fusion protein Ag33132 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 OTU domain, ubiquitin aldehyde binding 1
别名 OTU domain-containing ubiquitin aldehyde-binding protein 1, OTB1, HSPC263, hOTU1, EC:3.4.19.12
计算分子量 31 kDa and 35 kDa
观测分子量31-35 kDa
GenBank蛋白编号BC007519
基因名称 OTUB1
Gene ID (NCBI) 55611
RRIDAB_3085200
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96FW1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for OTUB1 antibody 68489-1-IgDownload protocol
IF protocol for OTUB1 antibody 68489-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols