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OTUD1 Polyclonal antibody

OTUD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29921-1-AP

Print datasheet

Synonyms

OTU domain containing 1, OTDC1, DUBA7, DUBA 7

验证数据展示

  • IHC staining of human cervical cancer using 29921-1-AP

    IHC staining of human cervical cancer using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29921-1-AP

    WB analysis using 29921-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29921-1-AP

    WB analysis using 29921-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 29921-1-AP

    IHC staining of human ovary tumor using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 29921-1-AP

    IHC staining of mouse liver using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 29921-1-AP

    IHC staining of human cervical cancer using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue, A549 cells
Positive IHC detected inhuman cervical cancer tissue, human ovary tumor tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29921-1-AP targets OTUD1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen OTUD1 fusion protein Ag31996 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OTU domain containing 1
Synonyms OTU domain containing 1, OTDC1, DUBA7, DUBA 7
Calculated Molecular Weight51 kDa
Observed Molecular Weight50-55 kDa
Gene Symbol OTUD1
Gene ID (NCBI) 220213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VV17
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).

实验方案

Product Specific Protocols
WB protocol for OTUD1 antibody 29921-1-APDownload protocol
IHC protocol for OTUD1 antibody 29921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer

Authors - Yurou Chen
View Article

Int J Cardiol

BECN1 regulates FADD/RIPK1/Caspase-8 complex formation via RIPK1 ubiquitination by downregulating OTUD1 in MI/R induced myocyte apoptosis

Authors - Lu Hongquan
View Article