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验证数据展示

  • WB analysis of HeLa using 29622-1-AP

    WB analysis of HeLa using 29622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 29622-1-AP

    IHC staining of mouse cerebellum using 29622-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OTUD3 Polyclonal antibody

OTUD3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29622-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

DUBA4, KIAA0459, OTU domain containing 3, OTUD3, RP11 460G22.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 750 μg/ml

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经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, HT-29 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29622-1-AP targets OTUD3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 OTUD3 fusion protein Ag30425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain containing 3
别名 DUBA4, KIAA0459, OTU domain containing 3, OTUD3, RP11 460G22.1
计算分子量 45 kDa
观测分子量43 kDa
GenBank蛋白编号NM_015207
基因名称 OTUD3
Gene ID (NCBI) 23252
RRIDAB_2918332
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T2D3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).

实验方案

Product Specific Protocols
WB protocol for OTUD3 antibody 29622-1-APDownload protocol
IHC protocol for OTUD3 antibody 29622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols