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验证数据展示

  • WB analysis of HeLa using 29622-1-AP

    WB analysis of HeLa using 29622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 29622-1-AP

    IHC staining of mouse cerebellum using 29622-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OTUD3 Polyclonal antibody

OTUD3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29622-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

DUBA4, KIAA0459, OTU domain containing 3, OTUD3, RP11 460G22.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, HT-29 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29622-1-AP targets OTUD3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen OTUD3 fusion protein Ag30425 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OTU domain containing 3
Synonyms DUBA4, KIAA0459, OTU domain containing 3, OTUD3, RP11 460G22.1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberNM_015207
Gene Symbol OTUD3
Gene ID (NCBI) 23252
RRIDAB_2918332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T2D3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).

实验方案

Product Specific Protocols
WB protocol for OTUD3 antibody 29622-1-APDownload protocol
IHC protocol for OTUD3 antibody 29622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols