验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25070-1-AP targets OTUD4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | OTUD4 fusion protein Ag18401 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | OTU domain containing 4 |
| 别名 | DUBA6, HIN1, HIV 1 induced protein HIN 1, HSHIN1, KIAA1046, OTU domain containing 4, OTUD4 |
| 计算分子量 | 1113 aa, 124 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC118653 |
| 基因名称 | OTUD4 |
| Gene ID (NCBI) | 54726 |
| RRID | AB_2879882 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01804 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTUD4, a member of the OTU family, encodes for a protein of 495 amino acids with an OTU domain and functions via their enzymatic deubiquitinating activities. OTUD4 nominally encodes a K48-specific deubiquitinase previously shown to counteract proteasomal degradation of DNA repair proteins. OTUD4 has been identified to be down-regulated in multiple human tumor types, and lower OTUD4 expression indicates a poor prognosis.(PMID: 36411454,PMID: 32184655,PMID: 29395066)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD4 antibody 25070-1-AP | Download protocol |
| IHC protocol for OTUD4 antibody 25070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors | ||
Front Microbiol MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway.
| ||
Exp Cell Res Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1
| ||
PLoS Pathog Non-canonical regulation of the reactivation of an oncogenic herpesvirus by the OTUD4-USP7 deubiquitinases | ||
Cell Death Dis OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway
| ||
Biochem Biophys Res Commun NRF2 regulates EGF stability through OTUD4 in lung adenocarcinoma |
