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验证数据展示

  • WB analysis of MCF-7 using 24486-1-AP

    WB analysis of MCF-7 using 24486-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24486-1-AP (OTUD6A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24486-1-AP

    IF Staining of HEK-293 using 24486-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using OTUD6A antibody (24486-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

OTUD6A Polyclonal antibody

OTUD6A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24486-1-AP

产品说明书

引用文献(10)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IF/ICC, ELISA and More (3)

别名

DUBA 2, DUBA2, HSHIN6, OTU domain containing 6A, OTUD6A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24486-1-AP targets OTUD6A in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IP, IF, CoIP
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 OTUD6A fusion protein Ag21639 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain containing 6A
别名 DUBA 2, DUBA2, HSHIN6, OTU domain containing 6A, OTUD6A
计算分子量 288 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC137356
基因名称 OTUD6A
Gene ID (NCBI) 139562
RRIDAB_2879567
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L8S5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OTUD6A antibody 24486-1-APDownload protocol
IF protocol for OTUD6A antibody 24486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP

Cell Death Differ

Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.

Authors - Yan Zhao
View Article
humanIF

Mol Oncol

Deubiquitinase OTUD6A promotes proliferation of cancer cells via regulating Drp1 stability and mitochondrial fission.

Authors - Le Shi
  • KD Validated
View Article
humanWB

Int J Mol Sci

OTUD6A Is an Aurora Kinase A-Specific Deubiquitinase.

Authors - Hyo Jin Kim
View Article
humanWB

Commun Biol

OTUD6A promotes prostate tumorigenesis via deubiquitinating Brg1 and AR.

Authors - Xuhong Fu
View Article
humanWB

FEBS Lett

OTUD6A orchestrates complex modulation of TEAD4-mediated transcriptional programs

Authors - Hyo Jin Kim
View Article

Int J Oncol

Ubiquitin-specific peptidase 5 and ovarian tumor deubiquitinase 6A are differentially expressed in p53+/+ and p53-/- HCT116 cells.

Authors - Soo-Yeon Kim
View Article