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  • Featured Product
  • KD/KO Validated

OTUD6A Polyclonal antibody

OTUD6A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 24486-1-AP

Print datasheet

Synonyms

DUBA 2, DUBA2, HSHIN6, OTU domain containing 6A, OTUD6A

验证数据展示

  • WB analysis of MCF-7 using 24486-1-AP

    WB analysis of MCF-7 using 24486-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24486-1-AP (OTUD6A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24486-1-AP

    IF Staining of HEK-293 using 24486-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using OTUD6A antibody (24486-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24486-1-AP targets OTUD6A in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen OTUD6A fusion protein Ag21639 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OTU domain containing 6A
Synonyms DUBA 2, DUBA2, HSHIN6, OTU domain containing 6A, OTUD6A
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC137356
Gene Symbol OTUD6A
Gene ID (NCBI) 139562
RRIDAB_2879567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L8S5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OTUD6A antibody 24486-1-APDownload protocol
IF protocol for OTUD6A antibody 24486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP

Cell Death Differ

Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.

Authors - Yan Zhao
View Article
humanIF

Mol Oncol

Deubiquitinase OTUD6A promotes proliferation of cancer cells via regulating Drp1 stability and mitochondrial fission.

Authors - Le Shi
  • KD Validated
View Article
humanWB

Int J Mol Sci

OTUD6A Is an Aurora Kinase A-Specific Deubiquitinase.

Authors - Hyo Jin Kim
View Article
humanWB

Commun Biol

OTUD6A promotes prostate tumorigenesis via deubiquitinating Brg1 and AR.

Authors - Xuhong Fu
View Article

Int J Oncol

Ubiquitin-specific peptidase 5 and ovarian tumor deubiquitinase 6A are differentially expressed in p53+/+ and p53-/- HCT116 cells.

Authors - Soo-Yeon Kim
View Article

Viruses

Deubiquitinase OTUD6A Regulates Innate Immune Response via Targeting UBC13

Authors - Zhiwei Li
View Article