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验证数据展示

  • WB analysis using 25430-1-AP

    WB analysis using 25430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25430-1-AP

    WB analysis of HeLa using 25430-1-AP

    WB result of OTUD6B antibody (25430-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD6B transfected HeLa cells.

  • IF Staining of PC-3 using 25430-1-AP

    IF Staining of PC-3 using 25430-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25430-1-AP (OTUD6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 25430-1-AP

    IHC staining of human ovary cancer using 25430-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 25430-1-AP (OTUD6B antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

OTUD6B Polyclonal antibody

OTUD6B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25430-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

DUBA-5, DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25430-1-AP targets OTUD6B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 OTUD6B fusion protein Ag22044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain containing 6B
别名 DUBA-5, DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77
计算分子量 293 aa, 34 kDa
观测分子量 34-40 kDa
GenBank蛋白编号BC029760
基因名称 OTUD6B
Gene ID (NCBI) 51633
RRIDAB_2880075
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6M0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OTUD6B antibody 25430-1-APDownload protocol
IHC protocol for OTUD6B antibody 25430-1-APDownload protocol
IF protocol for OTUD6B antibody 25430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Cell Mol Life Sci

Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway

Authors - Yu Liu
  • KD Validated
View Article
humanIHC,IF

Cell Biol Int

OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2

Authors - Guoqiang Xing
  • KD Validated
View Article