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  • Featured Product
  • KD/KO Validated

OTUD6B Polyclonal antibody

OTUD6B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25430-1-AP

Print datasheet

Synonyms

CGI 77, DUBA 5, DUBA5, OTU domain containing 6B, OTUD6B

验证数据展示

  • WB analysis using 25430-1-AP

    WB analysis using 25430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25430-1-AP

    WB analysis of HeLa using 25430-1-AP

    WB result of OTUD6B antibody (25430-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD6B transfected HeLa cells.

  • IHC staining of human ovary tumor using 25430-1-AP

    IHC staining of human ovary tumor using 25430-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25430-1-AP (OTUD6B Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of PC-3 using 25430-1-AP

    IF Staining of PC-3 using 25430-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25430-1-AP (OTUD6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25430-1-AP targets OTUD6B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen OTUD6B fusion protein Ag22044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OTU domain containing 6B
Synonyms CGI 77, DUBA 5, DUBA5, OTU domain containing 6B, OTUD6B
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC029760
Gene Symbol OTUD6B
Gene ID (NCBI) 51633
RRIDAB_2880075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6M0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OTUD6B antibody 25430-1-APDownload protocol
IHC protocol for OTUD6B antibody 25430-1-APDownload protocol
IF protocol for OTUD6B antibody 25430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Cell Mol Life Sci

Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway

Authors - Yu Liu
  • KD Validated
View Article
humanIHC,IF

Cell Biol Int

OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2

Authors - Guoqiang Xing
  • KD Validated
View Article