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OXER1 Polyclonal antibody

OXER1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55202-1-AP

Print datasheet

Synonyms

G-protein coupled receptor R527, G-protein coupled receptor 170, GPR170, GPCR, G protein coupled receptor 170

验证数据展示

  • FC experiment of K-562 using 55202-1-AP

    FC experiment of K-562 using 55202-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug OXER1 Polyclonal antibody (55202-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of K-562 using 55202-1-AP

    WB analysis of K-562 using 55202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55202-1-AP (OXER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55202-1-AP

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 55202-1-AP

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55202-1-AP targets OXER1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxoeicosanoid (OXE) receptor 1
Synonyms G-protein coupled receptor R527, G-protein coupled receptor 170, GPR170, GPCR, G protein coupled receptor 170
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 43-46 kDa
GenBank Accession NumberNM_148962
Gene Symbol OXER1
Gene ID (NCBI) 165140
RRIDAB_10858396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDS5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OXER1, also named as GPR170 and TG1019, is a receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. It seems to be coupled to the G(i)/G(o), families of heteromeric G proteins. (PMID:12065583) This antibody is specific to OXER1.

实验方案

Product Specific Protocols
WB protocol for OXER1 antibody 55202-1-APDownload protocol
IHC protocol for OXER1 antibody 55202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Nuclear translocation of the membrane oxoeicosanoid/androgen receptor, OXER1: possible mechanisms involved

Authors - Panagiotis Malamos
View Article