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验证数据展示

  • FC experiment of K-562 using 55202-1-AP

    FC experiment of K-562 using 55202-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug OXER1 Polyclonal antibody (55202-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of K-562 using 55202-1-AP

    WB analysis of K-562 using 55202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55202-1-AP (OXER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55202-1-AP

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 55202-1-AP

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens).

OXER1 Polyclonal antibody

OXER1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55202-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

G-protein coupled receptor R527, G-protein coupled receptor 170, GPR170, GPCR, G protein coupled receptor 170

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55202-1-AP targets OXER1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oxoeicosanoid (OXE) receptor 1
别名 G-protein coupled receptor R527, G-protein coupled receptor 170, GPR170, GPCR, G protein coupled receptor 170
计算分子量 46 kDa
观测分子量 43-46 kDa
GenBank蛋白编号NM_148962
基因名称 OXER1
Gene ID (NCBI) 165140
RRIDAB_10858396
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDS5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OXER1, also named as GPR170 and TG1019, is a receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. It seems to be coupled to the G(i)/G(o), families of heteromeric G proteins. (PMID:12065583) This antibody is specific to OXER1.

实验方案

Product Specific Protocols
WB protocol for OXER1 antibody 55202-1-APDownload protocol
IHC protocol for OXER1 antibody 55202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Nuclear translocation of the membrane oxoeicosanoid/androgen receptor, OXER1: possible mechanisms involved

Authors - Panagiotis Malamos
View Article