验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, Neuro-2a cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13514-1-AP targets OXR1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | OXR1 fusion protein Ag4439 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxidation resistance 1 |
| 别名 | Nbla00307, oxidation resistance 1, Oxidation resistance protein 1, OXR1 |
| 计算分子量 | 758 aa, 85 kDa |
| 观测分子量 | 120-140 kDa |
| GenBank蛋白编号 | BC032710 |
| 基因名称 | OXR1 |
| Gene ID (NCBI) | 55074 |
| RRID | AB_2877956 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N573 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Oxidation resistance protein 1 (OXR1) belongs to the OXR1 family. The major function of OXR1 is to control the expression of genes that alleviate oxidative stress by increasing cellular resistance to reactive oxygen species (ROS) and the stress these molecules cause the cell. OXR1's ability to reduce oxidative stress and neurodegeneration in multiple diseases strongly suggests that it can be an effective therapeutic target (PMID: 33384581).Biochemical experiments show that OXR1 inhibits V1-ATPase and causes disassembly of the holoenzyme, suggesting that OXR1 plays a direct role in V-ATPase regulation (PMID: 34918374). OXR1 has 8 isoforms with the molecular mass of 25, 28, 34, 56, 94, 95 and 98 kDa. Sometimes higher molecular weight around 120-140 kDa can also be observed, which may be a modified variant of OXR1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OXR1 antibody 13514-1-AP | Download protocol |
| IHC protocol for OXR1 antibody 13514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. | ||
J Inflamm Res Orexin-A Attenuates Inflammatory Responses in Lipopolysaccharide-Induced Neural Stem Cells by Regulating NF-KB and Phosphorylation of MAPK/P38/Erk Pathways. | ||
Front Mol Biosci 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis. | ||
Brain Res Bull Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism. | ||
Neurosci Bull MicroRNA-365 Knockdown Prevents Ischemic Neuronal Injury by Activating Oxidation Resistance 1-Mediated Antioxidant Signals. | ||
Redox Biol Iridium metal complex targeting oxidation resistance 1 protein attenuates spinal cord injury by inhibiting oxidative stress-associated reactive oxygen species |
