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  • Featured Product
  • KD/KO Validated

OXSR1 Polyclonal antibody

OXSR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15611-1-AP

Print datasheet

Synonyms

KIAA1101, OSR1, oxidative stress responsive 1, OXSR1

验证数据展示

  • IF Staining of MCF-7 using 15611-1-AP

    IF Staining of MCF-7 using 15611-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OXSR1 antibody (15611-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 15611-1-AP

    WB analysis using 15611-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15611-1-AP

    WB analysis of HeLa using 15611-1-AP

    WB result of OXSR1 antibody (15611-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXSR1 transfected HeLa cells.

  • IP experiment of HeLa using 15611-1-AP

    IP experiment of HeLa using 15611-1-AP

    IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IHC staining of human prostate cancer using 15611-1-AP

    IHC staining of human prostate cancer using 15611-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 15611-1-AP

    IHC staining of human prostate cancer using 15611-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, human liver tissue, human testis tissue, HeLa cells, Jurkat cells, U-251 cells
Positive IP detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15611-1-AP targets OXSR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen OXSR1 fusion protein Ag8002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxidative-stress responsive 1
Synonyms KIAA1101, OSR1, oxidative stress responsive 1, OXSR1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC008726
Gene Symbol OXSR1
Gene ID (NCBI) 9943
RRIDAB_2299030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132).

实验方案

Product Specific Protocols
WB protocol for OXSR1 antibody 15611-1-APDownload protocol
IHC protocol for OXSR1 antibody 15611-1-APDownload protocol
IF protocol for OXSR1 antibody 15611-1-APDownload protocol
IP protocol for OXSR1 antibody 15611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Bioengineered

Upregulation of Oxidative stress-responsive 1(OXSR1) Predicts Poor Prognosis and Promotes Hepatocellular Carcinoma Progression.

Authors - Jianhui Chen
View Article
ratWB,IF

Adv Sci (Weinh)

Modulation of Cerebrospinal Fluid Dysregulation via a SPAK and OSR1 Targeted Framework Nucleic Acid in Hydrocephalus

Authors - Qiguang Wang
View Article