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  • Featured Product
  • KD/KO Validated

Occludin Polyclonal antibody, PBS Only

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13409-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 13409-1-AP (same clone as 13409-1-PBS)

    WB analysis using 13409-1-AP (same clone as 13409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

    IP experiment of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

    IP result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

    IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

产品信息

13409-1-PBS targets Occludin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Occludin fusion protein Ag4057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name occludin
Synonyms
Calculated Molecular Weight 522 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2156308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16625
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).