Osteoprotegerin/TNFRSF11B Polyclonal antibody

Osteoprotegerin/TNFRSF11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31766-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

OCIF, OPG, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31766-1-AP targets Osteoprotegerin/TNFRSF11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Osteoprotegerin/TNFRSF11B fusion protein Eg0976 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 11b
别名 OCIF, OPG, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B
计算分子量46kDa
观测分子量56-60 kDa
GenBank蛋白编号NM_002546.4
基因名称 TNFRSF11B
Gene ID (NCBI) 4982
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO00300
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promotes bone formations. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

实验方案

Product Specific Protocols
WB protocol for Osteoprotegerin/TNFRSF11B antibody 31766-1-APDownload protocol
IHC protocol for Osteoprotegerin/TNFRSF11B antibody 31766-1-APDownload protocol
IF protocol for Osteoprotegerin/TNFRSF11B antibody 31766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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