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验证数据展示

  • WB analysis of human milk using 83506-1-RR

    WB analysis of human milk using 83506-1-RR

    human milk was subjected to SDS PAGE followed by western blot with 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 83506-1-RR

    IHC staining of rat kidney using 83506-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 83506-1-RR

    IHC staining of rat kidney using 83506-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83506-1-RR

    Affinity and Kinetic Characterization of 83506-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83506-1 against Human Osteoprotegerin/ TNFRSF11B were performed. The affinity constant is below 1 pM.

Osteoprotegerin/TNFRSF11B Recombinant antibody

Osteoprotegerin/TNFRSF11B Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83506-1-RR

产品说明书

CloneNo. 240168E9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG, OCIF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman milk
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83506-1-RR targets Osteoprotegerin/TNFRSF11B in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 Osteoprotegerin/TNFRSF11B fusion protein Ag2139 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 11b
别名 TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG, OCIF
计算分子量 401 aa, 46 kDa
观测分子量56 kDa
GenBank蛋白编号BC030155
基因名称 TNFRSF11B
Gene ID (NCBI) 4982
RRIDAB_3671131
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00300
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promotes bone formation. OPG plays a key role in cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

实验方案

Product Specific Protocols
WB protocol for Osteoprotegerin/TNFRSF11B antibody 83506-1-RRDownload protocol
IHC protocol for Osteoprotegerin/TNFRSF11B antibody 83506-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols