验证数据展示
经过测试的应用
Positive WB detected in | unboiled HeLa cells, unboiled HepG2 cells, SH-SY5Y cells |
Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | COLO 320 cells |
Boiling the sample after lysis is not recommended.
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67258-2-Ig targets P glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | P glycoprotein fusion protein Ag17811 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
别名 | ABCB1, 1G6C2, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1 |
计算分子量 | 1280 aa, 142 kDa |
观测分子量 | 130-150 kDa |
GenBank蛋白编号 | BC130424 |
基因名称 | P glycoprotein |
Gene ID (NCBI) | 5243 |
RRID | AB_3085039 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P08183 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection, we recommend to avoid boiling the sample after lysis.
实验方案
Product Specific Protocols | |
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WB protocol for P glycoprotein antibody 67258-2-Ig | Download protocol |
IHC protocol for P glycoprotein antibody 67258-2-Ig | Download protocol |
IF protocol for P glycoprotein antibody 67258-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |