• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse lung using 18002-1-AP

    WB analysis of mouse lung using 18002-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18002-1-AP (PP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 18002-1-AP

    IHC staining of human breast cancer using 18002-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18002-1-AP (PP11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 18002-1-AP

    IHC staining of human breast cancer using 18002-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18002-1-AP (PP11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 18002-1-AP

    IF Staining of HeLa using 18002-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18002-1-AP (PP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PP11 Polyclonal antibody

PP11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18002-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

26 serine protease, ENDOU, P11, Placental protein 11, PP11, Protein endoU, PRSS26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse lung tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

18002-1-AP targets PP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PP11 fusion protein Ag12494 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 26 serine protease
别名 26 serine protease, ENDOU, P11, Placental protein 11, PP11, Protein endoU, PRSS26
计算分子量 369 aa, 42 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC074763
基因名称 PP11
Gene ID (NCBI) 8909
RRIDAB_10598470
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21128
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PP11 antibody 18002-1-APDownload protocol
IHC protocol for PP11 antibody 18002-1-APDownload protocol
IF protocol for PP11 antibody 18002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Obstet Gynecol

Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.

Authors - Roni Levy
View Article
humanIHC

J Clin Biochem Nutr

Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.

Authors - Masataka Nomoto
View Article