验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy NOLC1 Ready-To-Use IHC Kit. NOLC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11815-1-AP targets NOLC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | NOLC1 fusion protein Ag2382 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleolar and coiled-body phosphoprotein 1 |
| 别名 | NS5ATP13, Nopp140, NOPP130, KIAA0035, Hepatitis C virus NS5A-transactivated protein 13 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC006769 |
| 基因名称 | NOLC1 |
| Gene ID (NCBI) | 9221 |
| RRID | AB_2235983 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14978 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs [PMID:21266110]. It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes [PMID:8972203].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOLC1 antibody 11815-1-AP | Download protocol |
| IHC protocol for NOLC1 antibody 11815-1-AP | Download protocol |
| IF protocol for NOLC1 antibody 11815-1-AP | Download protocol |
| IP protocol for NOLC1 antibody 11815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases | ||
Elife Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.
| ||
Acta Pharmacol Sin Atypical chemokine receptor 3 induces colorectal tumorigenesis in mice by promoting β-arrestin-NOLC1-fibrillarin-dependent rRNA biogenesis. | ||
Am J Cancer Res NOLC1 was identified as a tumor suppressor gene in thyroid cancer and correlated with prognosis by bioinformatics | ||
J Cell Sci Novel stress granules-like structures are induced via a paracrine mechanism during viral infection. |
