P16-INK4A Polyclonal antibody

P16-INK4A Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 30519-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, FC (Intra), IP, ELISA and More (1)

CDKN2A, MTS1, CDKN2, CDK4I, CDK4 inhibitor p16INK4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDKN2A/P16INK4A Ready-To-Use IHC Kit. CDKN2A/P16INK4A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30519-1-AP targets P16-INK4A in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 P16-INK4A fusion protein Ag29567 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 2A
别名 CDKN2A, MTS1, CDKN2, CDK4I, CDK4 inhibitor p16INK4
计算分子量 17 kDa
观测分子量 16 kDa
GenBank蛋白编号NM_000077
基因名称 CDKN2A
Gene ID (NCBI) 1029
RRIDAB_3086346
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).

实验方案

Product Specific Protocols
WB protocol for P16-INK4A antibody 30519-1-APDownload protocol
IP protocol for P16-INK4A antibody 30519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Cell Res

Suppression of GATA3 promotes epithelial-mesenchymal transition and simultaneous cellular senescence in human extravillous trophoblasts

Authors - En-Xiang Chen
mouseIHC

Aging Cell

Identifying ENO1 as a protein target of chlorogenic acid to inhibit cellular senescence and prevent skin photoaging in mice

Authors - Xueling He
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