验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IP detected in | HEK-293 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
30519-1-AP targets P16-INK4A in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | P16-INK4A fusion protein Ag29567 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cyclin-dependent kinase inhibitor 2A |
别名 | CDKN2A, MTS1, CDKN2, CDK4I, CDK4 inhibitor p16INK4 |
计算分子量 | 17 kDa |
观测分子量 | 16 kDa |
GenBank蛋白编号 | NM_000077 |
基因名称 | CDKN2A |
Gene ID (NCBI) | 1029 |
RRID | AB_3086346 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P42771 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).
实验方案
Product Specific Protocols | |
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WB protocol for P16-INK4A antibody 30519-1-AP | Download protocol |
IP protocol for P16-INK4A antibody 30519-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biochim Biophys Acta Mol Cell Res Suppression of GATA3 promotes epithelial-mesenchymal transition and simultaneous cellular senescence in human extravillous trophoblasts | ||
Aging Cell Identifying ENO1 as a protein target of chlorogenic acid to inhibit cellular senescence and prevent skin photoaging in mice |