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  • Featured Product
  • KD/KO Validated

CoraLite®647-conjugated P16-INK4A Polyclonal antibody

P16-INK4A Polyclonal Antibody for

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL650-10883

Print datasheet

Synonyms

ARF, ARF,CDKN2A, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, p14, p14ARF, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, P19, TP16

验证数据展示

For other applications, we recommend the unconjugated version of this antibody, 10883-1-AP

产品信息

CL650-10883 targets P16-INK4A in applications and shows reactivity with human samples.

Tested Applications Application Description
Tested Reactivity human
Immunogen P16-INK4A fusion protein Ag1328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Synonyms ARF, ARF,CDKN2A, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, p14, p14ARF, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, P19, TP16
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC021998
Gene Symbol P16-INK4A
Gene ID (NCBI) 1029
RRIDAB_2920359
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N726
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.

 What is the molecular weight of P16?

16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.

What is the function of p16?

P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).

 What is the role of p16 in senescence?

In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.

 What is the role of p16 in cancer?

As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).


实验方案

Product Specific Protocols
FC protocol for CL Plus 647 P16-INK4A antibody CL650-10883Download protocol
Standard Protocols
Click here to view our Standard Protocols