P21 Polyclonal antibody

P21 Polyclonal Antibody for WB, IF, ELISA
Cat No. 27296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat and More (2)

应用

WB, IF, ELISA and More (1)

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, PIC1, SDI1, WAF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNeuro-2a cells, C6 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDKN1A/P21 Ready-To-Use IHC Kit. CDKN1A/P21 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27296-1-AP targets P21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原 P21 fusion protein Ag26129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1A (p21, Cip1)
别名 CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, PIC1, SDI1, WAF1
计算分子量18 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000275
基因名称 p21
Gene ID (NCBI) 1026
RRIDAB_2880834
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP38936
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

实验方案

Product Specific Protocols
WB protocol for P21 antibody 27296-1-APDownload protocol
IF protocol for P21 antibody 27296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Am J Respir Crit Care Med

CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging

Authors - Huachun Cui
mouseWB

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
mouseWB

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
mouseIF

Cell Mol Life Sci

Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis

Authors - Fenglei Chen
mouseWB

Oxid Med Cell Longev

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.

Authors - Yanzhuo Zhang
mouseIF

Front Cell Dev Biol

P53 independent pathogenic mechanisms contribute to BubR1 microcephaly

Authors - Noelle A Sterling
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