验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
67355-1-Ig targets P27; KIP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | P27; KIP1 fusion protein Ag14634 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
| Synonyms | CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1 |
| Calculated Molecular Weight | 198 aa, 22 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC001971 |
| Gene Symbol | p27 Kip1/CDKN1B |
| Gene ID (NCBI) | 1027 |
| RRID | AB_2882610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P46527 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P27; KIP1 antibody 67355-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hepatocell Carcinoma CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 kip1 Axis | ||
Cell Rep FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation | ||
J Vasc Res Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis |
