P27; KIP1 Monoclonal antibody

P27; KIP1 Monoclonal Antibody for WB, ELISA
Cat No. 67355-1-Ig

产品说明书

CloneNo. 3F12C10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, ELISA

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CDKN1B Ready-To-Use IHC Kit. CDKN1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

67355-1-Ig targets P27; KIP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse
免疫原 P27; KIP1 fusion protein Ag14634 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1B (p27, Kip1)
别名 CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1
计算分子量 198 aa, 22 kDa
观测分子量 27 kDa
GenBank蛋白编号BC001971
基因名称 p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
RRIDAB_2882610
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP46527
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

实验方案

Product Specific Protocols
WB protocol for P27; KIP1 antibody 67355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Hepatocell Carcinoma

CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 kip1 Axis

Authors - Xin Zhou
humanWB

Cell Rep

FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation

Authors - Tongguan Tian
mouseWB

J Vasc Res

Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis

Authors - Wei Chang
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