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  • Featured Product
  • KD/KO Validated

P2RX4 Polyclonal antibody, PBS Only

P2RX4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13534-1-PBS

Print datasheet

Synonyms

Purinergic receptor, P2X4R, P2X4, P2X purinoceptor 4, ATP receptor

验证数据展示

  • WB analysis of HeLa using 13534-1-AP (same clone as 13534-1-PBS)

    WB analysis of HeLa using 13534-1-AP (same clone as 13534-1-PBS)

    WB result of P2RX4 antibody (13534-1-AP; 1:4000; room temperature for 1.5 hours) with sh-control and sh-P2RX4 transfected HeLa cells. This data was developed using the same antibody clone with 13534-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 13534-1-AP (same clone as 13534-1-PBS)

    WB analysis of HeLa using 13534-1-AP (same clone as 13534-1-PBS)

    WB result of P2RX4 antibody (13534-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and P2RX4 knockout HeLa cells. This data was developed using the same antibody clone with 13534-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 13534-1-AP (same clone as 13534-1-PBS)

    IHC staining of human ovary cancer using 13534-1-AP (same clone as 13534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13534-1-AP (P2RX4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13534-1-PBS in a different storage buffer formulation.

产品信息

13534-1-PBS targets P2RX4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen P2RX4 fusion protein Ag4445 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name purinergic receptor P2X, ligand-gated ion channel, 4
Synonyms Purinergic receptor, P2X4R, P2X4, P2X purinoceptor 4, ATP receptor
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC033826
Gene Symbol P2RX4
Gene ID (NCBI) 5025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439). The antibody can detect the band around 45 kDa.