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P2RX4 Monoclonal antibody, PBS Only

P2RX4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A6

Cat No : 66416-1-PBS

Print datasheet

Synonyms

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor

验证数据展示

  • WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

    WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

    IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

    IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

产品信息

66416-1-PBS targets P2RX4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen P2RX4 fusion protein Ag4445 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name purinergic receptor P2X, ligand-gated ion channel, 4
Synonyms ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC033826
Gene Symbol P2RX4
Gene ID (NCBI) 5025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).