• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

P2RY13 Polyclonal antibody

P2RY13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20335-1-AP

Print datasheet

Synonyms

G-protein coupled receptor 94, G-protein coupled receptor 86, GPR94, GPR86, GPCR1

验证数据展示

  • WB analysis of HeLa using 20335-1-AP

    WB analysis of HeLa using 20335-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20335-1-AP (P2RY13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20335-1-AP targets P2RY13 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen P2RY13 fusion protein Ag14108 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name purinergic receptor P2Y, G-protein coupled, 13
Synonyms G-protein coupled receptor 94, G-protein coupled receptor 86, GPR94, GPR86, GPCR1
Calculated Molecular Weight 354 aa, 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC041116
Gene Symbol P2RY13
Gene ID (NCBI) 53829
RRIDAB_2878674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P2RY13 (also named GPR86 or GPR94) is a G protein-coupled receptor (GPCR), which is involved in the pathogenesis of inflammation and immune disorders (PMID: 35982893). Several studies have reported that P2RY13 is a key regulator of cholesterol transport and hepatic HDL endocytosis and is involved in bone formation, remodeling, cell survival, and neuroprotection (PMID: 38045624).

实验方案

Product Specific Protocols
WB protocol for P2RY13 antibody 20335-1-APDownload protocol
IHC protocol for P2RY13 antibody 20335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Health Sci Rep

P2RY13 is a prognostic biomarker and associated with immune infiltrates in renal clear cell carcinoma: A comprehensive bioinformatic study

Authors - Jie Chu
View Article
humanWB

Nat Methods

SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes

Authors - Ruiqiao He
View Article