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  • Featured Product
  • KD/KO Validated

P4HA1 Monoclonal antibody, PBS Only

P4HA1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H10A6

Cat no : 66101-1-PBS

Print datasheet

Synonyms

Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6

验证数据展示

  • WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA1 antibody included
  • IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA1 antibody included
Planning an IHC experiment? We recommend our IHCeasy P4HA1 Ready-To-Use IHC Kit. P4HA1 primary antibody included.

产品信息

66101-1-PBS targets P4HA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen P4HA1 fusion protein Ag17867 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prolyl 4-hydroxylase, alpha polypeptide I
Synonyms Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6
Calculated Molecular Weight 534 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC034998
Gene Symbol P4HA1
Gene ID (NCBI) 5033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13674
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.