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  • Featured Product
  • KD/KO Validated

P4HA2 Monoclonal antibody, PBS Only

P4HA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9A4

Cat no : 66604-1-PBS

Print datasheet

Synonyms

4 PH alpha 2, P4HA2

验证数据展示

  • WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA2 antibody included
  • IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA2 antibody included
Planning an IHC experiment? We recommend our IHCeasy P4HA2 Ready-To-Use IHC Kit. P4HA2 primary antibody included.

产品信息

66604-1-PBS targets P4HA2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse , Rat
Immunogen P4HA2 fusion protein Ag4714 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prolyl 4-hydroxylase, alpha polypeptide II
Synonyms 4 PH alpha 2, P4HA2
Calculated Molecular Weight 533 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC035813
Gene Symbol P4HA2
Gene ID (NCBI) 8974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15460
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.