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P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31403-1-AP

Print datasheet

Synonyms

P62/SQSTM1, p62, Sqstm1, sequestosome 1, Osi

验证数据展示

  • WB analysis using 31403-1-AP

    WB analysis using 31403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31403-1-AP

    WB analysis using 31403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 31403-1-AP

    IF Staining of HepG2 using 31403-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using P62,SQSTM1 antibody (31403-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse brain tissue, RAW 264.7 cells
Positive IF/ICC detected inChloroquine treated HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31403-1-AP targets P62,SQSTM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen P62,SQSTM1 fusion protein Ag35064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sequestosome 1
Synonyms P62/SQSTM1, p62, Sqstm1, sequestosome 1, Osi
Calculated Molecular Weight48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberNM_011018
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 18412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ64337
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

实验方案

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols