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验证数据展示

  • WB analysis using 31403-1-AP

    WB analysis using 31403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31403-1-AP

    WB analysis using 31403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 31403-1-AP

    IP experiment of NIH/3T3 using 31403-1-AP

    IP result of anti-P62,SQSTM1 (IP:31403-1-AP, 4ug; Detection:31403-1-AP 1:5000) with NIH/3T3 cells lysate 1920 ug.

  • IF Staining of HepG2 using 31403-1-AP

    IF Staining of HepG2 using 31403-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using P62,SQSTM1 antibody (31403-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 31403-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

P62/SQSTM1, p62, Sqstm1, Sequestosome-1, sequestosome 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse brain tissue, RAW 264.7 cells
Positive IP detected inNIH/3T3 cells
Positive IF/ICC detected inChloroquine treated HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

31403-1-AP targets P62,SQSTM1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen P62,SQSTM1 fusion protein Ag35064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sequestosome 1
Synonyms P62/SQSTM1, p62, Sqstm1, Sequestosome-1, sequestosome 1
Calculated Molecular Weight48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberNM_011018
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 18412
RRIDAB_3669966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ64337
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

实验方案

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
IP protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Colorectal Dis

AQP3 mediates autophagy through SIRT1/p62 signal to alleviate intestinal epithelial cell damage caused by sepsis

Authors - Canmin Wang
View Article