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验证数据展示

  • WB analysis using 30092-1-AP

    WB analysis using 30092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30092-1-AP

    WB analysis using 30092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

P85a; PIK3R1 Polyclonal antibody

P85a; PIK3R1 Polyclonal Antibody for WB, ELISA
Cat No. 30092-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30092-1-AP targets P85a; PIK3R1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
别名 GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1
计算分子量 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC030815
基因名称 PI3 Kinase p85 Alpha
Gene ID (NCBI) 5295
RRIDAB_3086227
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27986
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for P85a; PIK3R1 antibody 30092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols