验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HEK-293 cells, rat spleen tissue, HL-60 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15348-1-AP targets PA2G4 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PA2G4 fusion protein Ag7587 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proliferation-associated 2G4, 38kDa |
| 别名 | hG4-1, HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 42 kDa, 48 kDa |
| GenBank蛋白编号 | BC001951 |
| 基因名称 | PA2G4 |
| Gene ID (NCBI) | 5036 |
| RRID | AB_2156602 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ80 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| IHC protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| IF protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| IP protocol for PA2G4 antibody 15348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biosci PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.
| ||
FASEB J Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency | ||
Methods Enzymol Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells | ||
J Dent Sci Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma | ||
Nat Commun Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML | ||
Appl Immunohistochem Mol Morphol Increased PA2G4 Expression Is an Unfavorable Factor in Nasopharyngeal Carcinoma |
