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  • Featured Product
  • KD/KO Validated

PA2G4 Polyclonal antibody

PA2G4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15348-1-AP

Print datasheet

Synonyms

hG4-1, HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1

验证数据展示

  • WB analysis of HEK-293 using 15348-1-AP

    WB analysis of HEK-293 using 15348-1-AP

    WB result of PA2G4 antibody (15348-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.

  • IHC staining of human breast cancer using 15348-1-AP

    IHC staining of human breast cancer using 15348-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PA2G4 antibody included
  • WB analysis using 15348-1-AP

    WB analysis using 15348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 15348-1-AP

    IP experiment of Jurkat using 15348-1-AP

    IP result of anti-PA2G4 (IP:15348-1-AP, 4ug; Detection:15348-1-AP 1:1000) with Jurkat cells lysate 3000ug.

  • IF Staining of HepG2 using 15348-1-AP

    IF Staining of HepG2 using 15348-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PA2G4 antibody (15348-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, rat spleen tissue, HL-60 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15348-1-AP targets PA2G4 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PA2G4 fusion protein Ag7587 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proliferation-associated 2G4, 38kDa
Synonyms hG4-1, HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 42 kDa, 48 kDa
GenBank Accession NumberBC001951
Gene Symbol PA2G4
Gene ID (NCBI) 5036
RRIDAB_2156602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ80
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.

实验方案

Product Specific Protocols
WB protocol for PA2G4 antibody 15348-1-APDownload protocol
IHC protocol for PA2G4 antibody 15348-1-APDownload protocol
IF protocol for PA2G4 antibody 15348-1-APDownload protocol
IP protocol for PA2G4 antibody 15348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,RIP

Cell Biosci

PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.

Authors - Sheng Sun
  • KD Validated
View Article
humanWB,IHC

FASEB J

Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency

Authors - Roseanne Rosario
View Article

Methods Enzymol

Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells

Authors - Manuel Günnigmann
View Article
humanWB,IF,IP

J Dent Sci

Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma

Authors - Legang Sun
View Article
humanWB

Nat Commun

Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML

Authors - Matteo Marchesini
View Article
humanIHC

Appl Immunohistochem Mol Morphol

Increased PA2G4 Expression Is an Unfavorable Factor in Nasopharyngeal Carcinoma

Authors - Yan Xu
View Article