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PAAF1 Polyclonal antibody

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17650-1-AP

Print datasheet

Synonyms

PAAF, PAAF1, Protein G 16, Rpn14, WDR71

验证数据展示

  • WB analysis of Jurkat using 17650-1-AP

    WB analysis of Jurkat using 17650-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17650-1-AP

    IHC staining of human testis using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 17650-1-AP

    IHC staining of human testis using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 17650-1-AP

    IF Staining of HEK-293 using 17650-1-AP

    Immunofluorescent analysis of HEK-293 cells using 17650-1-AP (PAAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, mouse brain tissue, human brain tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17650-1-AP targets PAAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PAAF1 fusion protein Ag11892 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasomal ATPase-associated factor 1
Synonyms PAAF, PAAF1, Protein G 16, Rpn14, WDR71
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC028628
Gene Symbol PAAF1
Gene ID (NCBI) 80227
RRIDAB_2878424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRP4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PAAF1 antibody 17650-1-APDownload protocol
IHC protocol for PAAF1 antibody 17650-1-APDownload protocol
IF protocol for PAAF1 antibody 17650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

PMID: 31285951

Authors - Wen-Si Zhao
View Article
humanIHC

Elife

Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes

Authors - Aojia Zhuang
View Article