验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, human brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse kidney tissue, human brain tissue, human kidney tissue, human testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
17650-1-AP targets PAAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PAAF1 fusion protein Ag11892 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasomal ATPase-associated factor 1 |
| 别名 | WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1 |
| 计算分子量 | 375 aa, 40 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC028628 |
| 基因名称 | PAAF1 |
| Gene ID (NCBI) | 80227 |
| RRID | AB_2878424 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRP4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAAF1 antibody 17650-1-AP | Download protocol |
| IHC protocol for PAAF1 antibody 17650-1-AP | Download protocol |
| IF protocol for PAAF1 antibody 17650-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes |
