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验证数据展示

  • WB analysis using 30172-1-AP

    WB analysis using 30172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HCT 116 using 30172-1-AP

    WB analysis of HCT 116 using 30172-1-AP

    WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells.

  • IP experiment of HCT 116 using 30172-1-AP

    IP experiment of HCT 116 using 30172-1-AP

    IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug.

  • IF Staining of HCT 116 using 30172-1-AP

    IF Staining of HCT 116 using 30172-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PACS2 Polyclonal antibody

PACS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 30172-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF/ICC, IP, ELISA

别名

FLJ25488, KIAA0602, PACS 2, PACS1 like protein, PACS1L, PACS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, NIH/3T3 cells, HuH-7 cells
Positive IP detected inHCT 116 cells
Positive IF/ICC detected inHCT 116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30172-1-AP targets PACS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 PACS2 fusion protein Ag32902 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphofurin acidic cluster sorting protein 2
别名 FLJ25488, KIAA0602, PACS 2, PACS1 like protein, PACS1L, PACS2
计算分子量98kd
观测分子量100-130 kDa
GenBank蛋白编号NM_001100913
基因名称 PACS2
Gene ID (NCBI) 23241
RRIDAB_3086254
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

实验方案

Product Specific Protocols
WB protocol for PACS2 antibody 30172-1-APDownload protocol
IF protocol for PACS2 antibody 30172-1-APDownload protocol
IP protocol for PACS2 antibody 30172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols