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PACSIN3 Polyclonal antibody

PACSIN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10639-1-AP

Print datasheet

Synonyms

SH3 domain-containing protein 6511, Protein kinase C and casein kinase substrate in neurons protein 3

验证数据展示

  • WB analysis of HeLa using 10639-1-AP

    WB analysis of HeLa using 10639-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10639-1-AP

    IHC staining of human breast cancer using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).

  • IF Staining of HeLa using 10639-1-AP

    IF Staining of HeLa using 10639-1-AP

    Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10639-1-AP targets PACSIN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PACSIN3 fusion protein Ag1006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C and casein kinase substrate in neurons 3
Synonyms SH3 domain-containing protein 6511, Protein kinase C and casein kinase substrate in neurons protein 3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC007914
Gene Symbol PACSIN3
Gene ID (NCBI) 29763
RRIDAB_2159453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PACSIN3 antibody 10639-1-APDownload protocol
IHC protocol for PACSIN3 antibody 10639-1-APDownload protocol
IF protocol for PACSIN3 antibody 10639-1-APDownload protocol
FC protocol for PACSIN3 antibody 10639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

Biallelic truncating variants in PACSIN3 cause childhood-onset myopathy with hyperCKaemia

Authors - Felix Distelmaier
View Article