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验证数据展示

  • WB analysis using 28636-1-AP

    WB analysis using 28636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PACT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28636-1-AP

    WB analysis of HeLa using 28636-1-AP

    WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected HeLa cells.

  • WB analysis of MCF-7 using 28636-1-AP

    WB analysis of MCF-7 using 28636-1-AP

    WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected MCF-7 cells.

  • Featured Product
  • KD/KO验证

PACT Polyclonal antibody

PACT Polyclonal Antibody for WB, ELISA
Cat No. 28636-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, MCF-7 cells, rat restis tissue, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy PRKRA Ready-To-Use IHC Kit. PRKRA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28636-1-AP targets PACT in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PACT fusion protein Ag29784 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, interferon-inducible double stranded RNA dependent activator
别名 DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC009470
基因名称 PRKRA
Gene ID (NCBI) 8575
RRIDAB_2918186
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75569
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

实验方案

Product Specific Protocols
WB protocol for PACT antibody 28636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols