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PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29169-1-AP

Print datasheet

Synonyms

KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II

验证数据展示

  • WB analysis using 29169-1-AP

    WB analysis using 29169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29169-1-AP (PADI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29169-1-AP

    IHC staining of human kidney using 29169-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29169-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29169-1-AP targets PADI2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PADI2 fusion protein Ag29042 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidyl arginine deiminase, type II
Synonyms KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II
Calculated Molecular Weight 665 aa, 75 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC009701
Gene Symbol PADI2
Gene ID (NCBI) 11240
RRIDAB_2918243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2J8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

实验方案

Product Specific Protocols
WB protocol for PADI2 antibody 29169-1-APDownload protocol
IHC protocol for PADI2 antibody 29169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols