验证数据展示
经过测试的应用
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
18187-1-AP targets PAEP/Glycodelin in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | PAEP/Glycodelin fusion protein Ag12902 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | progestagen-associated endometrial protein |
| 别名 | Glycodelin, PAEP, PAEG, GdS, GdF |
| 计算分子量 | 180 aa, 21 kDa |
| 观测分子量 | 24-28 kDa |
| GenBank蛋白编号 | BC069562 |
| 基因名称 | PAEP/Glycodelin |
| Gene ID (NCBI) | 5047 |
| RRID | AB_2918059 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09466 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycodelin (also known as Progestagen Associated Endometrial Protein, PAEP, and Placental Protein 14, PP14) is a secreted human lipocalin-family protein mainly expressed in well-differentiated epithelial cells in human reproductive tissues, like endometrium and seminal vesicles. While different cells produce differentially glycosylated isoforms of Glycodelin: decidualized endometrium-derived glycodelin-A (GdA), seminal vesicle-derived glycodelin-S (GdS), glycodelin-F (isolated from follicular fluid), and glycodelin-C (isolated from cumulus cells). Glycodelin has been involved in cell differentiation and tumor growth.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAEP/Glycodelin antibody 18187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |