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验证数据展示

  • WB analysis using 29304-1-AP

    WB analysis using 29304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29304-1-AP (PAFAH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29304-1-AP

    IHC staining of human prostate cancer using 29304-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29304-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PAFAH Polyclonal antibody

PAFAH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29304-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29304-1-AP targets PAFAH in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PAFAH fusion protein Ag30947 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
别名 Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7
计算分子量 50 kDa
观测分子量29-30 kDa, 45-55 kDa
GenBank蛋白编号BC038452
基因名称 PLA2G7
Gene ID (NCBI) 7941
RRIDAB_2918276
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13093
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet-activating factor acetylhydrolase (PAFAH) Ib consists of homo- or heterodimers of two PLA2 subunits α1 and α2, and a dimer of a third subunit, LIS1. PAFAH Ib was first discovered by its ability to hydrolyze the extracellular signaling lipid, PAF, which plays a role in platelet activation and inflammation. LIS1, the causative agent of the fatal brain disorder Miller-Dieker lissencephaly, functions independently of the α subunits to regulate the activity of cytoplasmic dynein. LIS1 is a highly conserved protein involved in dynein-mediated processes including nuclear and neuronal migration, centrosomal function, and mitotic spindle orientation. (PMID: 20624900, PMID: 23262398)

实验方案

Product Specific Protocols
WB protocol for PAFAH antibody 29304-1-APDownload protocol
IHC protocol for PAFAH antibody 29304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols