• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PAFAH Polyclonal antibody

PAFAH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29304-1-AP

Print datasheet

Synonyms

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7

验证数据展示

  • WB analysis using 29304-1-AP

    WB analysis using 29304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29304-1-AP (PAFAH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29304-1-AP

    IHC staining of human prostate cancer using 29304-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29304-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29304-1-AP targets PAFAH in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PAFAH fusion protein Ag30947 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Synonyms Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7
Calculated Molecular Weight 50 kDa
Observed Molecular Weight29-30 kDa, 45-55 kDa
GenBank Accession NumberBC038452
Gene Symbol PLA2G7
Gene ID (NCBI) 7941
RRIDAB_2918276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13093
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet-activating factor acetylhydrolase (PAFAH) Ib consists of homo- or heterodimers of two PLA2 subunits α1 and α2, and a dimer of a third subunit, LIS1. PAFAH Ib was first discovered by its ability to hydrolyze the extracellular signaling lipid, PAF, which plays a role in platelet activation and inflammation. LIS1, the causative agent of the fatal brain disorder Miller-Dieker lissencephaly, functions independently of the α subunits to regulate the activity of cytoplasmic dynein. LIS1 is a highly conserved protein involved in dynein-mediated processes including nuclear and neuronal migration, centrosomal function, and mitotic spindle orientation. (PMID: 20624900, PMID: 23262398)

实验方案

Product Specific Protocols
WB protocol for PAFAH antibody 29304-1-APDownload protocol
IHC protocol for PAFAH antibody 29304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols