PAFAH2 Polyclonal antibody
PAFAH2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
10085-1-AP targets PAFAH2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | PAFAH2 fusion protein Ag0124 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | platelet-activating factor acetylhydrolase 2, 40kDa |
| Synonyms | HSD PLA2, PAFAH2, SD PLA2 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 44-46 kDa |
| GenBank Accession Number | BC001158 |
| Gene Symbol | PAFAH2 |
| Gene ID (NCBI) | 5051 |
| RRID | AB_2160055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99487 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The family of platelet-activating factor-acetylhydrolase (PAF-AH) consists of enzymes which catalyze the hydrolysis of acetyl ester at the sn-2 position of PAF(Platelet-activating factor). The intracellular type II PAF-AH is a single polypeptide about a 40 kd distributed in both the cytosol and membranes of many kinds of cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| IHC protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| IP protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis. | ||
J Lipid Res Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis. | ||
Biochemistry Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress. |