• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse brain using 25029-1-AP

    WB analysis of mouse brain using 25029-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25029-1-AP (PAG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25029-1-AP

    IHC staining of human ovary cancer using 25029-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25029-1-AP (PAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PAG1 Polyclonal antibody

PAG1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25029-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, PAG, Csk-binding protein, Csk binding protein, CBP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25029-1-AP targets PAG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PAG1 fusion protein Ag13393 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoprotein associated with glycosphingolipid microdomains 1
别名 Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, PAG, Csk-binding protein, Csk binding protein, CBP
计算分子量 432 aa, 47 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC112159
基因名称 PAG1
Gene ID (NCBI) 55824
RRIDAB_2879858
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAG1, also named as CBP and PAG, promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It is involved in cell adhesion signaling. PAG1 may play an important role in inhibiting the proliferation, invasion and metastasis of the cancer cells. PAG1 is a membrane protein with phosphor modification. The MW of PAG1 migrates to 50-65 kDa in WB detection.

实验方案

Product Specific Protocols
WB protocol for PAG1 antibody 25029-1-APDownload protocol
IHC protocol for PAG1 antibody 25029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols