• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Serpin E1/PAI-1 Monoclonal antibody, PBS Only

Serpin E1/PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4A5

Cat no : 66261-1-PBS

Print datasheet

Synonyms

PAI-1, SERPINE1, SERPINE1, PAI-1, Plasminogen activator inhibitor 1, PAI1

验证数据展示

  • WB analysis of human peripheral blood platelets using 66261-1-Ig (same clone as 66261-1-PBS)

    WB analysis of human peripheral blood platelets using 66261-1-Ig (same clone as 66261-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

    IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINE1 antibody included
  • IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

    IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINE1 antibody included
  • IF Staining of HepG2 using 66261-1-Ig (same clone as 66261-1-PBS)

    IF Staining of HepG2 using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (66261-1-Ig, Clone: 1H4A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SERPINE1 Ready-To-Use IHC Kit. SERPINE1 primary antibody included.

产品信息

66261-1-PBS targets Serpin E1/PAI-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen Serpin E1/PAI-1 fusion protein Ag22411 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Synonyms PAI-1, SERPINE1, SERPINE1, PAI-1, Plasminogen activator inhibitor 1, PAI1
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC010860
Gene Symbol PAI-1
Gene ID (NCBI) 5054
RRIDAB_2881648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05121
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.