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PAIP1 Polyclonal antibody

PAIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 10675-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, IF/ICC, IP, ELISA and More (4)

Polyadenylate-binding protein-interacting protein 1, Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, BxPC-3 cells, COS-7 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10675-1-AP targets PAIP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP, RIP
经测试反应性 human, monkey
文献引用反应性human
免疫原 PAIP1 fusion protein Ag1070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly(A) binding protein interacting protein 1
别名 Polyadenylate-binding protein-interacting protein 1, Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1
计算分子量 70 kDa
观测分子量 60 kDa, 51 kDa, 40 kDa
GenBank蛋白编号BC005295
基因名称 PAIP1
Gene ID (NCBI) 10605
RRIDAB_2160391
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H074
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].

实验方案

Product Specific Protocols
WB protocol for PAIP1 antibody 10675-1-APDownload protocol
IF protocol for PAIP1 antibody 10675-1-APDownload protocol
IP protocol for PAIP1 antibody 10675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem J

Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.

Authors - Emma Jane Mead
humanWB,IHC,IF

Biol Reprod

Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2.

Authors - Yue He
humanWB,IHC

Hum Pathol

Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.

Authors - Yixuan Wang
  • KD Validated
humanWB,IF

Exp Cell Res

Role of Paip1 on angiogenesis and invasion in pancreatic cancer.

Authors - Hongjian Guan
  • KD Validated
humanWB,IHC

Cancer Res Treat

Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.

Authors - Nan Li
  • KD Validated
humanWB

Arch Biochem Biophys

YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1

Authors - Guozhi Yin
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