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  • Featured Product
  • KD/KO Validated

PAK1 Polyclonal antibody, PBS Only

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51137-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 51137-1-AP (same clone as 51137-1-PBS)

    WB analysis using 51137-1-AP (same clone as 51137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 51137-1-AP (same clone as 51137-1-PBS)

    WB analysis of HEK-293 using 51137-1-AP (same clone as 51137-1-PBS)

    WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAK1 antibody included
  • IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAK1 antibody included
  • IF Staining of HeLa using 51137-1-AP (same clone as 51137-1-PBS)

    IF Staining of HeLa using 51137-1-AP (same clone as 51137-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PAK1 Ready-To-Use IHC Kit. PAK1 primary antibody included.

产品信息

51137-1-PBS targets PAK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Synonyms
Calculated Molecular Weight 553 aa, 62 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC109299
Gene Symbol PAK1
Gene ID (NCBI) 5058
RRIDAB_2283523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13153
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.