验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, PC-3 cells, rat skin tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14685-1-AP targets PAK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PAK4 fusion protein Ag5981 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | p21 protein (Cdc42/Rac)-activated kinase 4 |
| 别名 | Serine/threonine-protein kinase PAK 4, PAK-4, PAK 4, p21-activated kinase 4, KIAA1142 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC025282 |
| 基因名称 | PAK4 |
| Gene ID (NCBI) | 10298 |
| RRID | AB_2158610 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O96013 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAK4 antibody 14685-1-AP | Download protocol |
| IHC protocol for PAK4 antibody 14685-1-AP | Download protocol |
| IF protocol for PAK4 antibody 14685-1-AP | Download protocol |
| IP protocol for PAK4 antibody 14685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.
| ||
Cancer Res Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma. | ||
Mol Ther Nucleic Acids ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway. | ||
Aging (Albany NY) PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.
| ||
Mol Neurobiol Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation |
