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验证数据展示

  • WB analysis using 27942-1-AP

    WB analysis using 27942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27942-1-AP (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 27942-1-AP

    IHC staining of human testis using 27942-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27942-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 27942-1-AP

    IHC staining of human testis using 27942-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27942-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PAK6 Polyclonal antibody

PAK6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27942-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, ELISA

别名

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27942-1-AP targets PAK6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性human
免疫原 PAK6 fusion protein Ag27687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 6
别名 p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6
计算分子量 681 aa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC035596
基因名称 PAK6
Gene ID (NCBI) 56924
RRIDAB_2881015
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the N-terminal of PAK6. This antibody is specific to PAK6.

实验方案

Product Specific Protocols
WB protocol for PAK6 antibody 27942-1-APDownload protocol
IHC protocol for PAK6 antibody 27942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharmacol Res

HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5

Authors - Fu-Chun Huo
View Article