验证数据展示
经过测试的应用
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12460-1-AP targets PAK4/6/7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PAK4/6/7 fusion protein Ag3127 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | p21 protein (Cdc42/Rac)-activated kinase 7 |
| 别名 | KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK4/6/7, PAK5, PAK7 |
| 计算分子量 | 719 aa, 81 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC024179 |
| 基因名称 | PAK7 |
| Gene ID (NCBI) | 57144 |
| RRID | AB_2877858 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P286 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. The antibody can recognize PAK4/6/7. PAK4/6/7 belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It acts on a variety of targets.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAK4/6/7 antibody 12460-1-AP | Download protocol |
| IP protocol for PAK4/6/7 antibody 12460-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |