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验证数据展示

  • WB analysis of mouse brain using 66961-1-Ig

    WB analysis of mouse brain using 66961-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 66961-1-Ig

    IF Staining of Neuro-2a using 66961-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PAK7 Monoclonal antibody

PAK7 Monoclonal Antibody for WB, IF, ELISA
Cat No. 66961-1-Ig

产品说明书

CloneNo. 1H3B6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IF, ELISA

别名

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IF/ICC detected inNeuro-2a cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66961-1-Ig targets PAK7 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 PAK7 fusion protein Ag27299 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 7
别名 KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7
计算分子量 719 aa, 81 kDa
观测分子量 80 kDa
GenBank蛋白编号BC024179
基因名称 PAK7
Gene ID (NCBI) 57144
RRIDAB_2882284
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 66961-1-IgDownload protocol
IF protocol for PAK7 antibody 66961-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols