验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, THP-1 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
14340-1-AP targets PALB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PALB2 fusion protein Ag5654 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | partner and localizer of BRCA2 |
| 别名 | PALB 2, FANCN |
| 计算分子量 | 131 kDa |
| 观测分子量 | 130 kDa; 80-90 kDa; 250-260 kDa |
| GenBank蛋白编号 | BC044254 |
| 基因名称 | PALB2 |
| Gene ID (NCBI) | 79728 |
| RRID | AB_2158774 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86YC2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PALB2 can interact with BRCA1 and BRCA2 and form the BRCA1-PALB2-BRCA2 complex which is critical for homologous recombination and DNA damage response. PALB2 is an essential partner of BRCA2 that promotes the localization and stability of BRCA2. The predicted MW of PALB2 is approximately 130 kDa, while the truncated PALB2 protein arround 80-90 kDa can also be detected in SDS-PAGE similar to papers. (PubMed:16793542, 24141787, 24153426). There's the dimer form with MW 250-260 kDa (refer to PMID: 30289697).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PALB2 antibody 14340-1-AP | Download protocol |
| IHC protocol for PALB2 antibody 14340-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region. | ||
Res Sq Haploinsufficiency of ZNF251 causes DNA-PKcs-dependent resistance to PARP inhibitors in BRCA1-mutated cancer cells | ||
Cancer Lett ZNF251 haploinsufficiency confers PARP inhibitors resistance in BRCA1-mutated cancer cells through activation of homologous recombination | ||
Molecules FOXO3a Mediates Homologous Recombination Repair (HRR) via Transcriptional Activation of MRE11, BRCA1, BRIP1, and RAD50 |