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验证数据展示

  • WB analysis of human heart using 66601-1-Ig

    WB analysis of human heart using 66601-1-Ig

    Human heart tissue, pig heart tissue and rat heart tissue were subjected to SDS PAGE followed by western blot with 66601-1-Ig (Palladin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66601-1-Ig

    IHC staining of human colon cancer using 66601-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66601-1-Ig (Palladin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 66601-1-Ig

    IF Staining of mouse testis using 66601-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PALLD,palladin antibody (66601-1-Ig, Clone: 2A1F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Palladin Monoclonal antibody

Palladin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66601-1-Ig

产品说明书

CloneNo. 2A1F6
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, pig and More (1)

应用

WB, IHC, IF-P, ELISA

别名

CGI 151, KIAA0992, palladin, PALLD, PALLD,palladin, PNCA1, Sarcoma antigen NY SAR 77, SIH002

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, pig heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66601-1-Ig targets Palladin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 Human, rat, pig
文献引用反应性human
免疫原 Palladin fusion protein Ag1288 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 palladin, cytoskeletal associated protein
别名 CGI 151, KIAA0992, palladin, PALLD, PALLD,palladin, PNCA1, Sarcoma antigen NY SAR 77, SIH002
计算分子量 1383 aa, 151 kDa
观测分子量 151 kDa
GenBank蛋白编号BC013867
基因名称 palladin
Gene ID (NCBI) 23022
RRIDAB_2881961
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WX93
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Palladin antibody 66601-1-IgDownload protocol
IHC protocol for Palladin antibody 66601-1-IgDownload protocol
IF protocol for Palladin antibody 66601-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling

Authors - Xiong Shu
View Article