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验证数据展示

  • WB analysis of HeLa using 16179-1-AP

    WB analysis of HeLa using 16179-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16179-1-AP (Palladin-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16179-1-AP

    IP experiment of HeLa using 16179-1-AP

    IP result of anti-Palladin-Specific (IP:16179-1-AP, 4ug; Detection:16179-1-AP 1:200) with HeLa cells lysate 1500ug.

Palladin-Specific Polyclonal antibody

Palladin-Specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 16179-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16179-1-AP targets Palladin-Specific in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 palladin, cytoskeletal associated protein
别名 CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002
计算分子量 151 kDa
观测分子量 120 kDa
GenBank蛋白编号NM_016081
基因名称 palladin
Gene ID (NCBI) 23022
RRIDAB_10695626
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WX93
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kDa, 140 kDa, and 65 kDa isoforms of this protein.

实验方案

Product Specific Protocols
WB protocol for Palladin-Specific antibody 16179-1-APDownload protocol
IP protocol for Palladin-Specific antibody 16179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols