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验证数据展示

  • WB analysis of HeLa using 16179-1-AP

    WB analysis of HeLa using 16179-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16179-1-AP (Palladin-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16179-1-AP

    IP experiment of HeLa using 16179-1-AP

    IP result of anti-Palladin-Specific (IP:16179-1-AP, 4ug; Detection:16179-1-AP 1:200) with HeLa cells lysate 1500ug.

Palladin-Specific Polyclonal antibody

Palladin-Specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 16179-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16179-1-AP targets Palladin-Specific in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name palladin, cytoskeletal associated protein
Synonyms CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberNM_016081
Gene Symbol palladin
Gene ID (NCBI) 23022
RRIDAB_10695626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WX93
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kDa, 140 kDa, and 65 kDa isoforms of this protein.

实验方案

Product Specific Protocols
WB protocol for Palladin-Specific antibody 16179-1-APDownload protocol
IP protocol for Palladin-Specific antibody 16179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols