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PAM Polyclonal antibody

PAM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26972-1-AP

Print datasheet

Synonyms

PAL, PAM, Peptidylamidoglycolate lyase, PHM

验证数据展示

  • WB analysis of U2OS using 26972-1-AP

    WB analysis of U2OS using 26972-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 26972-1-AP (PAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 26972-1-AP

    IHC staining of human colon using 26972-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26972-1-AP (PAM Antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inU2OS cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26972-1-AP targets PAM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhamster
Immunogen PAM fusion protein Ag25719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylglycine alpha-amidating monooxygenase
Synonyms PAL, PAM, Peptidylamidoglycolate lyase, PHM
Calculated Molecular Weight 108 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC018127
Gene Symbol PAM
Gene ID (NCBI) 5066
RRIDAB_2880706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PAM antibody 26972-1-APDownload protocol
IHC protocol for PAM antibody 26972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
hamsterWB

Comp Biochem Physiol B Biochem Mol Biol

Change on apoptosis, autophagy and mitochondria of the Harderian gland in Cricetulus barabensis during age.

Authors - Xiao-Tong Kong
View Article