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PAM16 Polyclonal antibody

PAM16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15321-1-AP

Print datasheet

Synonyms

MAGMAS, CGI-136, TIM16, Presequence translocated-associated motor subunit PAM16, Mitochondrial import inner membrane translocase subunit TIM16

验证数据展示

  • WB analysis of HepG2 using 15321-1-AP

    WB analysis of HepG2 using 15321-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15321-1-AP (PAM16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 15321-1-AP

    IHC staining of human ovary cancer using 15321-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15321-1-AP (PAM16 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15321-1-AP targets PAM16 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PAM16 fusion protein Ag0970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction
Synonyms MAGMAS, CGI-136, TIM16, Presequence translocated-associated motor subunit PAM16, Mitochondrial import inner membrane translocase subunit TIM16
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC005024
Gene Symbol PAM16
Gene ID (NCBI) 51025
RRIDAB_2878126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3D7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAM16, also named as Magmas, TIM16 and TIMM16, is regulates ATP-dependent protein translocation into the mitochondrial matrix. The MW of PAM16 is about 14-16 kDa in WB detection. PAM16 is a component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19.

实验方案

Product Specific Protocols
WB protocol for PAM16 antibody 15321-1-APDownload protocol
IHC protocol for PAM16 antibody 15321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy

Authors - Jonas Benjamin Michaelis
View Article
humanIHC

J Neurooncol

Magmas inhibition as a potential treatment strategy in malignant glioma.

Authors - Kaijun Di
View Article
mouseWB

Redox Biol

Lead aggravates Alzheimer's disease pathology via mitochondrial copper accumulation regulated by COX17

Authors - Dingbang Huang
View Article