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验证数据展示

  • WB analysis of mouse skeletal muscle using 55310-1-AP

    WB analysis of mouse skeletal muscle using 55310-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55310-1-AP (PAMR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 55310-1-AP

    IHC staining of human skeletal muscle using 55310-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55310-1-AP (PAMR1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 55310-1-AP

    IHC staining of human skeletal muscle using 55310-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55310-1-AP (PAMR1 antibody) at dilution of 1:50 (under 40x lens).

PAMR1 Polyclonal antibody

PAMR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55310-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DKFZP586H2123, FP938, Inactive serine protease PAMR1, PAMR1, RAMP, UNQ699/PRO1344

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55310-1-AP targets PAMR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regeneration associated muscle protease
别名 DKFZP586H2123, FP938, Inactive serine protease PAMR1, PAMR1, RAMP, UNQ699/PRO1344
计算分子量 82 kDa
观测分子量 78-82 kDa, 67-70 kDa
GenBank蛋白编号NM_015430
基因名称 PAMR1
Gene ID (NCBI) 25891
RRIDAB_11232034
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXH9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAMR1,also named as RAMP, belongs to the peptidase S1 family. It may play a role in regeneration of skeletal muscle. This antibody is specific to PAMR1.

实验方案

Product Specific Protocols
WB protocol for PAMR1 antibody 55310-1-APDownload protocol
IHC protocol for PAMR1 antibody 55310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Elife

The subiculum is a patchwork of discrete subregions.

Authors - Mark S Cembrowski
View Article
humanWB, IHC

Front Oncol

High Expression of PAMR1 Predicts Favorable Prognosis and Inhibits Proliferation, Invasion, and Migration in Cervical Cancer.

Authors - Rui Yang
View Article
human,mouseWB

J Ethnopharmacol

Qizhu Anticancer Prescription enhances immunosurveillance of liver cancer cells by regulating p21-dependent secretory phenotypes

Authors - Rui Hu
View Article