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验证数据展示

  • WB analysis using 25655-1-AP

    WB analysis using 25655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25655-1-AP (PAN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25655-1-AP

    IHC staining of human ovary cancer using 25655-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25655-1-AP (PAN3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAN3 antibody included

PAN3 Polyclonal antibody

PAN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25655-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Poly(A)-nuclease deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, PAN deadenylation complex subunit 3, PAB1P-dependent poly(A)-specific ribonuclease, hPan3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAN3 Ready-To-Use IHC Kit. PAN3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25655-1-AP targets PAN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PAN3 fusion protein Ag22559 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
Synonyms Poly(A)-nuclease deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, PAN deadenylation complex subunit 3, PAB1P-dependent poly(A)-specific ribonuclease, hPan3
Calculated Molecular Weight 887 aa, 96 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC128180
Gene Symbol PAN3
Gene ID (NCBI) 255967
RRIDAB_3669491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ58A45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform2.

实验方案

Product Specific Protocols
WB protocol for PAN3 antibody 25655-1-APDownload protocol
IHC protocol for PAN3 antibody 25655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols