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验证数据展示

  • WB analysis using 25655-1-AP

    WB analysis using 25655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25655-1-AP (PAN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25655-1-AP

    IHC staining of human ovary cancer using 25655-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25655-1-AP (PAN3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAN3 antibody included

PAN3 Polyclonal antibody

PAN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25655-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Poly(A)-nuclease deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, PAN deadenylation complex subunit 3, PAB1P-dependent poly(A)-specific ribonuclease, hPan3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAN3 Ready-To-Use IHC Kit. PAN3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25655-1-AP targets PAN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PAN3 fusion protein Ag22559 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
别名 Poly(A)-nuclease deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, PAN deadenylation complex subunit 3, PAB1P-dependent poly(A)-specific ribonuclease, hPan3
计算分子量 887 aa, 96 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC128180
基因名称 PAN3
Gene ID (NCBI) 255967
RRIDAB_3669491
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ58A45
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform2.

实验方案

Product Specific Protocols
WB protocol for PAN3 antibody 25655-1-APDownload protocol
IHC protocol for PAN3 antibody 25655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols